Genetic and epigenetic effects of salinity on in vitro growth of barley
نویسندگان
چکیده
Morphological, physiological and molecular changes were investigated in in vitro salt-stressed barley (Hordeum vulgare L. cv. Tokak). Mature embryos were cultured in Murashige and Skoog medium containing 0 (control), 50 and 100 mM NaCl for 20 days. Both concentrations inhibited shoot growth, decreased fresh weight and protein content, and increased SOD (EC 1.15.1.1) activity in a dose-dependent manner. The lower concentration increased root growth. Salinity caused nucleotide variations in roots, but did not affect shoot DNAs. The higher concentration caused methylation changes, mainly hypermethylation in shoots. This is the first study on genetic and epigenetic effects of salinity in barley.
منابع مشابه
Genetic Analysis of Agronomic Traits of Barley (Hordeum vulgare L.) Cultivars under Salinity Stress using Diallel Cross
In order to determine the heritability and genetic parameters of some agronomic traits in barely (Hordeum vulgare L.) cultivars, a seven-parent half diallel (F1 crosses + parents) was conducted in the non-stress and salt stress (8 and 12 ds m-1) conditions in a randomized complete block design with three replications. Genetic analysis was performed by Hayman’s method and Griffing’s fixed model,...
متن کاملEffect of salinity stress on physiological characteristics and protein profile of tolerant and sensitive barley (Hordeum vulgare L.) cultivars at vegetative growth stage
To study the variation of plant traits related to stress tolerance in tolerant and sensitive barley cultivars under salt stress conditions, an experiment was carried out as factorial arrangements in completely randomized design in Payame Noor University of Mahabad, Iran in 2018. Application of salinity stress of 250 mM of sodium chloride started from tillering stage, and leaf samples were taken...
متن کاملبررسی غلظت سدیم و نسبت پتاسیم به سدیم به‌عنوان ملاک تحمل به شوری در گندم و جو
Most researches on wheat and barley breeding for salt tolerance have focused mainly on excluding Na+ from different tissues but the results of some experiments suggest that contribution of Na+ exclusion to salt tolerance is overshadowed by other physiological responses. Three bread wheat cultivars differing in salt tolerance (Arg, Tajan and Baharan) and one barley cultivar (Nik) were employed t...
متن کاملEffects of salinity stress and Zinc application and some physiological traits on grain filling of three barley cultivars
To evaluate the effect of salinity stress and Zinc application on grain filling of three barley cultivars and some physiological traits this this experiment was arranged as a split block in a randomized complete block design with three replications at the Esfahan (Rodasht) salinity research station (2013-14). Three water quality treatments including (2, 10 and 18 dS/m) were evaluated in vertica...
متن کاملImproving barley performance by proper foliar applied salicylic-acid under saline conditions
Despite general effect of salicylic-acid (SA) in improving plant growth andproductivity in saline conditions, there have not been unanimity about the bestconcentration. In this 2-yr field study the effect of different SA concentrations(0, 0.5, 1.0, 1.5 and 2.0 mM) was examined on growth, grain yield and yieldcomponents of barley under two non-saline (2 dS m-1) and saline (12 dS m-1)conditions. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 36 شماره
صفحات -
تاریخ انتشار 2013